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Fig. 2. Cx43 increases the susceptibility of LNCaP cells to TNF ${alpha}$ -induced cell death. (A) PC3 cells were left uninfected ( ${triangledown}$ ) or infected with Ad-Control ( $bullet$ ) or Ad-Cx43 ( ${blacksquare}$ ) for 12 hours and then treated with 0.01-100 ng/ml TNF ${alpha}$ for 48 hours. (B) LNCaP cells were left uninfected ( ${triangledown}$ ) or infected with Ad-Control ( $bullet$ ) or Ad-Cx43 ( ${blacksquare}$ ) for 12 hours and then treated with 0.01-100 ng/ml TNF ${alpha}$ for 48 hours. Cell viability of untreated and Ad-Control infected cells showed a slight, but insignificant decline with increasing doses of TNF ${alpha}$ . These curves did not differ significantly from each other. Ad-Cx43-infected cells showed significantly less viability at 0.1, 1, 10, and 100 ng/ml TNF ${alpha}$ (P<0.0001). (C) LNCaP cells were infected with Ad-Control ( $bullet$ ), Ad-Cx43 ( ${blacksquare}$ ), or both Ad-Cx43 and Ad-Cx43DN ( ${diamond}$ ) for 12 hours and then treated with 0.01-100 ng/ml TNF ${alpha}$ for 48 hours. The viability of Ad-Control- or Ad-Cx43 + Ad-Cx43DN-infected cells did not differ from each other. The viability of cells infected with Ad-Cx43 was significantly lower than that of cells infected with both viruses at 0.1-100 ng/ml TNF ${alpha}$ (P=0.0264 for 0.1 ng/ml; P<0.0001 for 1.0, 10, and 100 ng/ml). The results are presented as mean ± s.e.m. although in many cases the error bars are so small that they are obscured by the symbols.

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