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Figure 5


Fig. 5. TNF{alpha} induces the death of Cx43-expressing LNCaP cells through TNFR1. (A) Immunoblots showing levels of TNFR1, TNFR2 and beta-actin in uninfected LNCaP cells (Control), LNCaP cells 12 hours after infection with Ad-Cx43 (Ad-Cx43), uninfected LNCaP cells after 12 hours of treatment with 10 ng/ml TNF{alpha} (TNF{alpha}), and LNCaP cells 12 hours after Ad-Cx43 infection and TNF{alpha} treatment (Ad-Cx43 + TNF{alpha}). (B) Bar graph showing viability of Cx43-expressing LNCaP cells in the presence or absence of neutralizing anti-TNFR1 (Ab-R1) or –TNFR2 (Ab-R2) antibodies (concentrations indicated in µg/ml). Cell viability determined by MTS assay is expressed as a percentage of control values. Statistical analysis of the raw absorbance data showed that treatment with 1 or 10 µg/ml of anti-TNFR1 antibodies or the combination of 10 µg/ml anti-TNFR1 and 10 µg/ml anti-TNFR2 antibodies significantly antagonized the effects of TNF{alpha} (P<0.0001 when compared to the viability of cells treated with TNF{alpha} alone).





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