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Files in this Data Supplement:
Fig. S1. Cos7 cells (A) and HeLa cells (B) were transfected with a plasmid for expression of LPR1 with a C-terminal HA tag. Ha tagged proteins and actin were visualized by fluorescence microscopy.
Fig. S2. HeLa cells were transfected with plasmids for expression of LPR1 mutants: EGFP-LPR1 S198T, EGFP-LPR1 H200G, EGFP-LPR1 R246E (A), as well as the C-terminal mutant, EGFP-LPR1 C-term Δ43 (B). Cells were analyzed by fluorescence microscopy to determine the localization of EGFP tagged proteins and actin. C. Untransfected (control) HeLa cells, or HeLa cells transfected with either EGFP-LPR1 or EGFP-LPR1 C-term Δ43 were examined by fluorescent microscopy, and peripheral filopodia were quantitated as described in the text. Twenty-five cells were counted in each category and mean numbers of filpodia ± s.d. were plotted. (D) HeLa cells were transfected with vectors for expression of EGFP, EGFP-LPP3, EGFP-LPR1, and three mutants of EGFP-LPR1, described in the text. Cell lysates were generated and analyzed by western blotting for expression of EGFP-tagged proteins with a GFP-specific antibody.
Fig. S3. HeLa cells were transfected with a vector for expression of myc-tagged constitutively active cdc42 (cdc42 61L). Myc-tagged protein and endogenously expressed Vasp were visualized by fluorescence microscopy.
Movie 1. HeLa cells expressing EGFP-LPR1, fluorescence. Frames were captured every 5 seconds; the video frame rate is 10 frames/second.
Movie 2. HeLa cells expressing EGFP-LPR1, phase. Frames were captured every 5 seconds; the video frame rate is 10 frames/second.
Movie 3. HeLa cells expressing EGFP-LPR1, fluorescence. Frames were captured every 5 seconds; the video frame rate is 10 frames/second.
Movie 4. HeLa cells expressing EGFP-LPR1, phase. Frames were captured every 5 seconds; the video frame rate is 10 frames/second.
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