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Figure 6


Fig. 6. LPR1-labeled filopodia have a unique protein composition. HeLa cells were transfected with pEGFP-LPR1, and immunofluorescence microscopy was used to visualize EGFP-tagged LPR1 and different endogenously expressed proteins: paxillin (A), Vasp (B) and myosin X (C). (D) Cos7 cells were transfected with vectors for expression of EGFP-LPR1 and localization of endogenous fascin and EGFP-LPR1 were analyzed by immunofluorescence microscopy. Fascin labels intracellular structures as well as membrane protrusions. High magnification images of the periphery of the cells show how these markers localize exclusively within the filopodia. Bars, 10 µm.





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