spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. BAD-LAMP is present in vesicles clustering in specific areas of the neurons. Immunofluorescence confocal microscopy of cortical neurons. (A) Staining for BAD-LAMP (red) and cholera toxin (GM1, green). (B) Staining for BAD-LAMP (red) and PrP (green). (C) Staining for BAD-LAMP (red) and N-CAM (green). BAD-LAMP is expressed in small vesicles clustered in neurites and accumulates in areas lacking surface semi-ordered lipid microdomain resident proteins (arrowheads). (D) THY1 labelling (green) defines the zones in which BAD-LAMP vesicles accumulate (red). However, THY1 (red) is present at the cell surface and does not co-localize with BAD-LAMP as seen at higher magnification (bottom panels, arrowheads). (E) Cholesterol depletion disrupts cluster organization and induces BAD-LAMP (red) and cholera toxin (GM1, green) co-localization. Bars, 20 µm; 10 µm for THY1 high magnification.





Right arrow Return to article