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Figure 6


Fig. 6. Confocal immunofluorescence microscopy analysis of BAD-LAMP transport in cortical neurons. (A) Staining for LAMP2 (green) and BAD-LAMP (red). (B) Internalized transferrin-FITC (green) in early and recycling endosomes and BAD-LAMP (red). (C) Staining for Ti-VAMP (green) and BAD-LAMP (red). (D) Staining of a growth cone for synaptotagmin 1 (SYT1, green) and BAD-LAMP (red). (E) Staining of a growth cone for VAMP2 (green) and BAD-LAMP (red). (F) Staining for syntaxin 6 (green) and BAD-LAMP (red). BAD-LAMP does not display any significant co-localization with LAMP1 and internalized transferrin. Bars, 20 µm in A,B,C,F, 10 µm in D,E.





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