spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Surface biotinylation reveals the endocytic nature of BAD-LAMP-containing vesicles. Cortical neurons were surface biotinylated for 15 minutes at 4°C, prior to warming at 37°C for different times, fixation and visualization by confocal microscopy. (A) Prior to warming, no significant co-localization of biotinylated proteins with BAD-LAMP was observed. Co-localization was evaluated using the Image J image analysis software. A low Pearson's coefficient and strong negative pixel shift are both indicative of the absence of staining overlap (right). (B) After 5 minutes of endocytosis at 37°C, extensive co-localization of biotinylated proteins (green) was observed with BAD-LAMP (red) in neurites (arrowheads), as also shown by a higher Pearson's coefficient and the absence of pixel shift (right). (C) After 45 minutes of endocytosis co-localization of BAD-LAMP with biotin is decreased as shown by a decreased Pearson's coefficient and negative pixel shift (right). Bar, 10 µm.





Right arrow Return to article