JCS016626 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 (Adobe PDF) -

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• Supplemental Table S2 (Adobe PDF) -

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• Supplemental Figure S1 -

  Fig. S1. Developmental expression of alternate MCPH1 isoforms. (A,B) MCPH1 immunoblots. (A) Developmental western of wild-type extracts shows MCPH1 protein is present in a variety of tissues and at several life-cycle stages. Extracts of embryos and testes were relatively underloaded (loading control: anti-α-tubulin). (B) Type A and B MCPH1 isoforms produced in vitro co-migrate on SDS-PAGE with endogenous MCPH1 isoforms abundant in testes and ovaries, respectively.

• Supplemental Figure S2 -

  Fig. S2. MCPH1 is a nuclear protein. Wild-type syncytial embryos were fixed and stained with an antibody against MCPH1 (green) and DNA dye (red). Representative embryos in various cell-cycle stages are shown. MCPH1 localizes to the nucleus during interphase and prophase and is no longer detectable during later stages of mitosis (following nuclear envelope breakdown). No MCPH1 signal is detected in interphase nuclei of mcph1^Z1861 mutants (negative control). Bar, 10 μm.

• Supplemental Figure S3 -

  Fig. S3. Decreased γ-tubulin staining of centrosomes in mcph1 embryos. Syncytial embryos from wild-type or mcph1^Exc21 females were fixed and co-stained with antibodies against α-tubulin (red) and γ-tubulin (green). Representative mitotic spindles are shown. Bar, 10 μm.