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Fig. 1. The awol phenotype. Representative syncytial embryos (A,B) and mitotic spindles (C-K) in embryos from wild-type or awol^Z1861/awol^Z0978 females. (A,B) DNA staining of embryos from awol females shows arrest with condensed chromosomes and unevenly spaced nuclei (B) compared to wild type (A). (C-G) Microtubules are in green and DNA in red. (C) Asynchronous neighboring nuclei in embryo from awol female (left, interphase; right, mitosis). (D) Metaphase spindle with duplicated centrosomes in embryo from awol female shows asynchronous nuclear and centrosome cycles (duplication normally occurs in telophase). (E) Shortened, barrel-shaped spindle in embryo from awol female. (F) DNA displaced from metaphase plate is tethered by microtubules to spindle pole in embryo from awol female. (G) Wild-type spindle. (H-K) Microtubules are in green and centrosomes in red. (H-I) awol spindles with missing or ectopic centrosomes. (K) Wild-type spindle. Bars, 20 µm.

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