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Figure 3


Fig. 3. Suppression of mcph1 by Chk2 (mnk). (A-J) Representative mitotic spindles in syncytial embryos and whole-mount embryos from mcph1Z1861, mnk mcph1Z1861 and wild-type females. Bars, 20 µm. (A-F) Microtubules are in green and DNA in red; low (A,B) and high (C-F) magnification views. mcph1 embryos have awol-type (barrel-shaped, acentrosomal) spindles (A,C). awol phenotype is suppressed in mnk mcph1 embryos (B,D): note restoration of elongated spindles and attached centrosomes. Other defects are seen in mnk mcph1 embryos, such as DNA shared by two spindles (E) and DNA bridging (F, arrow). (G,H) Cellularized embryos (2-3 hours) stained for actin (green) and DNA (red). mnk mcph1 embryos reach gastrulation with irregular cell size and DNA content (G) compared to wild type (H). (I,J) DNA-stained embryos (3-4 hours). mnk mcph1 embryos (I) arrest peri-gastrulation with aberrant morphology compared to wild type (J). (K) Quantification of suppression of developmental arrest of mcph1Z1861 embryos by mnk.





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