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Fig. 5. mcph1 larvae have intact DNA checkpoints and normal sensitivity to DNA-damaging agents. (A,B) Cell-cycle checkpoints in mcph1 larvae. Bars, 50 µm. (A) G2-M checkpoint. Eye-antennal imaginal disks were dissected from untreated (left) or irradiated (right) larvae, fixed, and stained with antibodies against phosphorylated Histone H3 (anti-PH3), a marker of mitotic cells. Lack of anti-PH3 staining post-IR indicates G2 arrest. Representative disks are shown (with at least twelve discs scored per genotype). (B) Intra-S phase checkpoint. Brains were dissected from untreated (left) or irradiated (right) larvae and labeled with BrdU. Decreased BrdU staining in brain lobes (arrows) post-IR indicates intra-S phase arrest. Representative brains are shown (with at least six brains scored per genotype). (C,D) Survival of mcph1 larvae following exposure to DNA-damaging agents. (C) Sensitivity to hydroxyurea (HU). Larvae were grown on food minus or plus HU and allowed to develop. For each genotype, the ratio of homozygous mutant to total progeny is expressed as a percentage with total number of adult flies scored shown in parentheses. (D) Sensitivity to IR. Third instar larvae were untreated or exposed to low-dose irradiation and allowed to develop. For each genotype, the ratio of eclosed adults to total pupae is expressed as a percentage with total pupae shown in parentheses.
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