JCS014290 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. The mcph1(L) transcript is present in the Drosophila testis. RT-PCR of overlapping fragments of the mcph(L) transcript from testis RNA. (A) Schematic of PCR fragments (predicted sizes in brackets). (B) RT-PCR showing duplicate experiments of fragments 1 and 2 in lanes 1-4, and fragments 3-5 in lanes 5-7. All PCR products shown were sequenced and assembled, confirming the predicted mcph1(L) transcript sequence.

• Supplemental Figure S2 -

  Fig. S2. Alignment of human (H.s) and Drosophila (D.s.) Microcephalin. Amino acid sequence alignment of human and Drosophila Microcephalin. Black shading represents identical residues and grey shading indicate similar amino acids. The protein’s three BRCT domains are boxed

• Movie 1 -

  Movie 1.GFP-MCPH1(S) isoform localisation during mitosis in a blastoderm stage embryo.

• Movie 2 -

  Movie 2. GFP-MCPH1(L) isoform localisation during mitosis in a blastoderm stage embryo.