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Figure 3


Fig. 3. Mutant proteins Cx45.6(P88S) and Cx45.6(D47A) inhibit the intercellular coupling mediated by wild-type Cx45.6. CEF cells were co-infected with recombinant retroviruses RCAS-Cx45.6 and mutants RCAS(A)-Cx45.6(P88S) and RCAS(A)-Cx45.6(D47A). The extent of dye transfer was quantified by the scrape-loading dye-transfer assay using LY-RD (A) and parachuting dye-transfer assay using calcein-Dil (B). The data are presented as mean±s.e.m.; n=3. ***P<0.001, in comparison with the non-Cx45.6-overexpressing control.





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