spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Stimulation of lens differentiation by Cx56*45.6C but not by Cx45.6*56C. (A) Cx45.6*56C was generated by fusing Cx45.6 lacking its C-terminus with the C-terminus from Cx56. Cx56*45.6C was made by fusing Cx56 lacking its C-terminus with the C-terminus from Cx45.6. (B) Recombinant retroviruses containing cDNAs encoding wild-type Cx45.6 (lane 3), Cx45.6*56C (lane 1) and Cx56*45.6C (lane 2) or RCAS(A) vehicle control (lane 4) were infected into primary lens cultures. After 6 days of infection, lens cell lysates were analyzed by SDS-PAGE and immunoblotted with antibodies against FLAG or beta-actin. (C) RCAS(A) retrovirus containing Cx45.6, Cx56, Cx45*56C and Cx56*45.6C sequences were infected into CEF cells and intercellular coupling was determined by scrape-loading dye transfer using LY-RD; n=3. (D) 6 days after infection, the total lentoid numbers were quantified at various time points; n=3. (E) 6 days after injection, primary lens cells were labeled with antibody against MIP and detected by fluorescein-conjugated anti-mouse IgG. The percentage of areas with MIP expression versus whole-image areas was determined; n=5. The data are presented as the mean±s.e.m.





Right arrow Return to article