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Figure 7


Fig. 7. Stimulation of lens differentiation by the C-terminus of Cx45.6. (A) Cx45.6C contains the entire C-terminus of Cx45.6. Cx45.6(–C) is a truncated mutant without its C-terminus. (B) Recombinant retroviruses containing wild-type Cx45.6 (lane 1), Cx45.6C (lane 2) and Cx45.6(–C) (lane 3) were infected into primary lens cultures. After 6 days of infection, lens cell lysates were analyzed by SDS-PAGE and immunoblotted with antibodies against FLAG or beta-actin. (C) 6 days after infection, the total numbers of lentoids formed were quantified at various time points.





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