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Figure 4


Fig. 4. cla4{Delta}, erg4{Delta} and ncp1{Delta} cells have defects in cell wall assembly. (A) Deletion of ERG4 results in increased sensitivity to Calcofluor White. Serial dilutions (1:10) of wild-type and the indicated deletion strains were spotted on a YPD plate and a YPD plate supplemented with 50 µg/ml Calcofluor White. Cells were cultured for 2 days at 30°C. (B) cla4{Delta}, erg4{Delta} and ncp1{Delta} cells are hypersensitive to zymolyase. The indicated strains were grown for 16 hours at 30°C in the presence of various amounts (0-20 U/ml) of zymolyase, starting with an OD600 of 0.025. Various dilutions of the cell suspensions were then plated on YPD plates to determine the cell titer. Shown here is one of three representative experiments.





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