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Figure 1


Fig. 1. Tmod3 localizes to lateral membranes in several epithelial cell types, including Caco-2 cells where it is the sole Tmod isoform. (A-I) XY confocal sections of LLC-PK1 CL4 (A-C), HBE (D-F), and Caco-2 (G-I) cells stained for F-actin (red, B,E,H) and Tmod3 (green, C,F,I). Tmod3 localizes to lateral membranes (arrowheads) in each of these epithelial cell lines as well as in a punctate pattern in the cytoplasm. Note the localization of Tmod3 to foci in LLC-PK1 CL4 cells (C); we have no information regarding the identification of these foci. (J) Immunoblot of Caco-2 cell lysates, human erythrocyte membranes (Mg2+ ghosts), and purified recombinant human Tmod1 or Tmod3 labeled with either anti-Tmod3 or anti-Tmod1 antibodies, demonstrating that Tmod3 is the sole Tmod isoform expressed in Caco-2 cells. (K-M) XZ confocal section of Caco-2 cells stained for F-actin (red, L) and Tmod3 (green, M). Tmod3 localizes to the subapical domain underlying the F-actin rich microvilli (arrows), to lateral membranes (arrowheads) and to cytoplasmic puncta.





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