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Figure 3


Fig. 3. Tmod3 knockdown by shRNA results in decreased fluorescence intensity of tropomyosin. (A-D) XZ and (E-H) XY confocal sections stained for F-actin (red, B,F), tropomyosin (green, C,G), with transfected cells expressing GFP shown in blue (D,H). (I) Transfection of pEGFP-H1RNAi encoding a hairpin sequence directed against Tmod3 results in decreased total F-actin and tropomyosin fluorescence as compared with untransfected cells. Total cellular F-actin and tropomyosin fluorescence intensity were quantified for untransfected cells and cells transfected with either pEGFP-H1RNAi encoding a hairpin sequence directed against Tmod3 or with pEGFP-H1RNAi encoding a mismatched target sequence, from images such as in F-I. Results are the mean ± s.e.m.; *P<0.05. (J) Representative line scans illustrating typical decreases in intensity of lateral membrane peaks of F-actin and tropomyosin fluorescence, corresponding with increased GFP fluorescence in cells transfected with pEGFP-H1RNAi encoding hairpin sequence directed against Tmod3; 7.8 pixels per µm.





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