spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Tmod3 knockdown by shRNA does not disrupt adherens or tight junctions, as assessed by localization of E-cadherin or ZO-1, but leads to increased breadth of {alpha}II-spectrin staining on lateral membranes. (A) Schematic of typical localization of E-cadherin, ZO-1 and {alpha}II-spectrin in polarized epithelial cells. (B-M) XZ confocal sections of Caco-2 monolayers stained for F-actin (red, C,G,K), E-cadherin (D), ZO-1 (H), {alpha}II-spectrin (L) (all shown in green), with transfected cells expressing GFP shown in blue (E,I,M). (O,P) Transfection of pEGFP-H1RNAi encoding a hairpin sequence directed against Tmod3 results in increased breadth of {alpha}II-spectrin staining at lateral membranes when compared to (O) untransfected cells but has no effect on breadth of E-cadherin staining at (P) lateral membranes. No significant changes are observed in (O) {alpha}II-spectrin or (P) E-cadherin on lateral membranes for cells transfected with pEGFP-H1RNAi encoding mismatched target sequence as compared to untransfected cells. Results are the mean ± s.e.m.; **P<0.005.





Right arrow Return to article