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Fig. 1. An incomplete mode of cytokinesis takes place in the liver, triggered by weaning. (A) Immunostaining for 
-catenin (red) and -tubulin (green) of liver sections (10- and 25-day-old rats). Images of telophase were visualized taking into account condensed chromatin staining (Hoechst 33342, blue). Immunostaining allowed us to distinguish between telophase that completed or did not complete cytokinesis. The percentages of cells with complete cytokinesis are indicated. Bars, 3 µm. (B) Events of incomplete cytokinesis before and after weaning (fixed at 19 days, arrow). At each point, four rats were independently analyzed. Percentages of complete and incomplete cytokinesis in telophase cells were calculated using -catenin/-tubulin/Hoechst immunostaining (see A). A total of 50 telophase cells were analyzed per animal. Bars represent s.e., P<0.0001.
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