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Fig. 2. The GGA proteins direct the localization of scNpc2p. GFP-ALP (top), scNCR1-GFP (middle) and scNPC2-GFP (bottom) plasmids were transformed into gga1
gga2
deletion yeast cells. The GFP-tagged proteins were visualized by direct fluorescence microscopy. Arrows indicate areas of mislocalization. A magnification of the boxed area is depicted. The corresponding DIC images are shown.