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Fig. 4. The AP-3 complex directs the localization of scNcr1p and scNpc2p. GFP-ALP (top), scNCR1-GFP (middle) and scNPC2-GFP (bottom) plasmids were transformed into yeast deletions of the AP-3 complex (apl6
, apl5
, apm3
and aps3
). The GFP-tagged proteins were visualized by direct fluorescence microscopy. Arrows indicate areas of mislocalization. The corresponding DIC images are shown.