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Figure 5


Fig. 5. mNPC1 co-localizes with AP-3. AP-3+/+ and AP-3–/– mouse fibroblasts were infected with an adenovirus encoding mNPC1-GFP. The GFP-tagged protein (green) was assessed for co-distribution with AP-3 and with the various organellar markers [AP-1, LAMPI and syntaxin 8 (Syn8)] (red) by indirect confocal microscopy using an anti-GFP antibody. Co-distribution is visualized in yellow in the merged images. Boxed areas are shown magnified by 400% (Mag). Bar, 10 µm.





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