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Figure 7


Fig. 7. Mammalian NPC1 transport is dependent on AP-3. (A) Non-permeabilized AP-3+/+ and AP-3–/– mouse fibroblasts were biotinylated (Biot.) in order to label surface proteins. Immunoblot analysis of streptavidin-precipitated proteins was performed for endogenous mNPC1, LAMPI, TfR, caveolin 1 (Cav1) and beta actin. As a control, 2% of the total input is shown. (B) Quantification of mNPC1 surface content was determined by normalizing the (surface content genotype/input content genotype)/(surface content wild type/input content wild type) to Cav1. The white bars are AP-3+/+ and the gray are AP-3–/–. beta actin is shown as a loading control. Numbers in parentheses are the number of independent determinations. Standard deviations are indicated.





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