spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Flagellar localization of FAP133 requires anterograde IFT. (A) Flagella were isolated from wild-type (CC124) and fla10 cells (harbors a temperature-sensitive mutation in the FLA10 kinesin-2 gene) after incubation for 1 hour at either the permissive temperature of 22°C or the restrictive temperature of 32°C. Flagellar proteins were separated in a 5-15% polyacrylamide gel and either stained with Coomassie Blue (upper panel) or transferred to nitrocellulose and probed with antibodies against FAP133 and outer dynein arm protein IC1 (lower panels). FAP133 levels are reduced in the fla10 strain only at the restrictive temperature. (B) Wild-type and fla10 cells, under the same conditions as in (A), were fixed and processed for indirect immunofluorescence microscopy using the FAP133 antibody. The punctate FAP133 signal was absent only from the flagella of fla10 cells at the restrictive temperature.





Right arrow Return to article