JCS04119 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Detail of Arabidopsis TERT-V(I8) sequence. (A) Nucleotide sequence of TERT-V(I8) found in the screen after sequencing pGADT7-rec. Vector sequences are shown in capitals and underlined. The 5′UTR of AtTERT-V(I8) is shown in italics. Exon boundaries (last six nucleotides) are shown in bold and underlined. Non-spliced introns are highlighted in grey. The start and stop codons (atg and tga) are shown in bold. The poly A tail at the end of TERT-V(I8) is shown in italics. (B) The corresponding translated protein is given with the amino acids corresponding to the GQ motif in bold, the predicted NLS are in bold, highlighted in grey, and the amino acids corresponding to the retained intron 6 are underlined. The stop codon (*) appears 6 amino acids after the junction between exon 6 and intron 6.

• Supplemental Figure S2 -

  Fig. S2. Control GFP markers for different chromatin domains. The top panels show the GFP signal in green. The central panels show the DAPI staining, rendered in red and the Bottom panels show a merged image of GFP and DAPI where overlap appears yellow. AtH3-like-GFP localises to the nucleus and co-localise with euchromatin in BY-2 cells. Examples of an interphase (left) and a metaphase stages (right) are shown. Bar, 10 μm. AtCENH3-GFP marks heterochromatin knobs in interphase cells (example of a Col-0 cell) and highlights centromeric regions as shown on a dividing BY-2 cell. Bar, 5 μm.