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Figure 6


Fig. 6. Localisation of the Arabidopsis POT1 proteins. (A) POT1A, POT1B and KU80 localise both to the cytoplasm and to the nucleus of Arabidopsis Col-0 cells. Top panel: localisation of telomeric POT1A, POT1B and KU80 fused to GFP in living cells. Bottom panel: Arabidopsis Col-0 cells transiently transformed with POT1A, POT1B and KU80 fused to GFP were extracted with detergent. Localisation of the proteins is shown in the GFP panel. Arrows indicate nuclei. The chromatin was stained with DAPI (DAPI panel) and co-localisation of the signals obtained with GFP and DAPI are shown in the merge panel. (B) Nuclear localisation of TERT-V(I8) and of a TERT truncation fused to GFP in Arabidopsis Col-0 cells. The two GFP fusions label the nucleoli (arrows) and the entire nuclei in some cells. Bars, 4 µm.





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