spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. EGF ligand release can be quantitatively tuned across a wide dynamic range. (A) Ligand release rate is measured from the slope of EGF accumulation over time. After 16 hours in serum-free media, cells were switched to fresh serum-free media or to media containing an inhibitor and samples were collected at 2-hour time intervals from triplicate wells. EGF concentration was measured using an EGF ELISA. The approximate ligand release rates (molecules of EGF released/cell/minute), verified in independent experiments, are shown next to each linear fit. (B) TCT release into the media over an 8 hour time period is inhibited using increasing concentrations of batimastat. Fitting the conditioned EGF concentration to a dose-response curve reveals a median inhibition concentration of 0.33 µM batimastat. (C) Adding increasing concentrations of batimastat down-modulates the linear ligand release of TCT cells. Error bars represent one standard deviation from the mean of triplicate samples.





Right arrow Return to article