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Figure 2


Fig. 2. Depletion of RILP inhibits ligand-induced EGFR degradation as studied by western blotting. (A) HeLa cells treated with control RNA or siRNA against RILP or Vps22 were stimulated for 15, 60, 120 or 180 minutes with 50 ng/ml EGF in the presence of 10 µg/ml cycloheximide before the cells were lysed and analyzed by SDS-PAGE and western blotting with antibodies against EGFR. The same membrane was then reblotted with antibody against beta-tubulin to verify equal loadings. (B) The intensities of the EGFR staining were quantified and plotted as a percentage of the respective intensities after 15 minutes of EGF stimulation. The error bars represent the s.e.m. of three experiments. According to a Student's t-test, P<0.05 for the 120 and 180 minutes time points.





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