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Figure 3


Fig. 3. Depletion of RILP inhibits ligand-induced EGFR degradation as studied by confocal microscopy. HeLa cells treated with control RNA (`A'), siRNA against RILP (`B') or siRNA against Vps22 (`C') were pretreated with 10 µg/ml cycloheximide (1 hour) before they were stimulated for 15, 60, 120 or 180 minutes with 50 ng/ml EGF. (A) The cells were permeabilized before fixation and stained for EGFR (red). (B) The intensities of the EGFR staining were quantified using Zeiss LSM 510 software (version 3.2) and plotted as a percentage of the respective intensities after 15 minutes of EGF stimulation. The error bars represent the s.e.m. of three experiments. According to Student's t-test, significantly less EGFR was degraded in RILP- or Vps22-depleted cells compared with control RNA cells (P<0.05 for all time points).





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