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Fig. 5. Depletion of RILP decreases lysosomal degradation of CI-M6PR without affecting its trafficking from the TGN to endosomes. (A) The expression of CI-M6PR (red), TGN46 (green) and EEA1 (blue) was determined in HeLa cells treated with control RNA or siRNA against RILP by confocal immunofluorescence. (B) All the images were scanned at the same settings below saturation, and the intensities of CI-M6PR, TGN46 and EEA1 (relative to the control) were determined by the Zeiss LSM 510 software (version 3.2). The graph represents the average (±s.e.) of three individual experiments, where at least 15 cells were quantified per experiment. (C) The expression of CI-M6PR in control cells and RILP siRNA cells was determined by western blotting, using 
-tubulin as a loading control. (D) CI-M6PR expression (relative to -tubulin) was quantified from three separate western blot experiments (±s.e.). The quantifications in B and D showed significantly higher levels of CI-M6PR in RILP-depleted cells than in control cells according to a Student's t-test (P<0.05).
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