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Figure 5


Fig. 5. AMER1 controls intercellular junctions together with APC. (A) Immunofluorescence staining of E-cadherin in MCF-7 cells treated with a control siRNA against GFP (siGFP), siRNA against AMER1 (siAMER1c), or siAMER1c together with an siRNA against APC (siAMER1c+siAPC). Arrowheads indicate disrupted cell junctions. (B) Relative number of gaps between MCF-7 cells transfected with siRNA oligonucleotides as indicated below the bars. Gaps were counted in E-cadherin- and beta-catenin-stained samples in 20 optical fields using the 40x objective. Results show the mean±s.d. of at least two independent experiments.





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