QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 6. Overexpression of STIM1 rescues inhibition by nocodazole. To evaluate SOCE, changes in the intracellular Ca²⁺ concentration were monitored in HEK 293 cells in which Ca²⁺ stores were depleted with thapsigargin (Tg; 2 µM) in the presence of a nominally Ca²⁺-free extracellular solution, followed by addition of 1.8 mM Ca²⁺. In wild-type HEK 293 cells overexpressing unconjugated EYFP (A) and cells overexpressing EYFP-STIM1 (B), SOCE was evaluated following a 20 minute incubation in 10 µM nocodazole (NZL; red traces) or 0.1% DMSO (black traces). Each trace represents the average response of all cells on a single coverslip (20-30 cells). (C) The average difference between the peak 340/380 value following Ca²⁺ addition and the 340/380 value just before Ca²⁺ addition was calculated for EYFP-expressing cells treated with DMSO (n=89, three coverslips) or 10 µM nocodazole (n=110, four coverslips) and in EYFP-STIM1-expressing cells treated with DMSO (n=77, three coverslips) or with 10 µM nocodazole (n=84, 3 coverslips) for experiments performed as described in (A) and (B). The data are reported as the mean±s.e.m.; the P values are based on t-tests.

Return to article