QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 9. Colchicine potentiates but does not activate SOCE in cells overexpressing STIM1. (A) HEK 293 cells expressing EYFP-STIM1 were treated with 1.0 µM CPA alone (Control; black trace) or with CPA and 100 µM colchicine (red trace) for 20 minutes in a nominally Ca²⁺-free extracellular solution, followed by addition of 1.8 mM extracellular Ca²⁺ to reveal SOCE. Also shown are data from cells treated with colchicine alone (blue trace). Each trace represents the average response of all cells on a single coverslip (20-30 cells). (B) The average difference between the peak 340/380 value following Ca²⁺ addition and the 340/380 value just before Ca²⁺ addition was calculated for CPA-treated control (n=64, three coverslips) and colchicine-treated (n=97, three coverslips) cells for experiments performed as described in (A). The data are reported as the mean±s.e.m.; the P value is based on t-test.

Return to article