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Fig. 7. Effect of rapamycin on RyR-dependent Ca²⁺ signaling WT and RyR1^–/– urinary bladder myocytes. (A) Effects of rapamycin (1 µM) on 10 mM caffeine-induced Ca²⁺ responses in WT urinary bladder myocytes and in RyR1^–/– mouse myocytes. (B-D) Ca²⁺ responses induced by (B) 100 µM and (C) 50 µM rapamycin in WT or in (D) RyR1^–/– myocytes (D). Rapamycin was applied near the cell by pressure ejection from a glass pipette at the time indicated on the line-scan image. ^*P<0.05.

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