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Figure 2


Fig. 2. EGFP-supervillin (EGFP-SV) and myosin heavy chain (MHC) IIB co-localize at the cell periphery during spreading. (A,B) COS7 cells expressing EGFP-SV (upper panels) or EGFP only (lower panels) after 30 (A) or 60 (B) minutes of spreading were counterstained with anti-MHC IIB antibodies. EGFP signals (a,d; green in c,f) and MHC IIB signals visualized in the red channel (b,e; red in c,f) were merged (c,f); signal overlaps are shown in yellow. Arrows indicate peripheral co-localizations. Bar, 20 µm.





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