spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Supervillin binds to the S2 domains of both of the nonmuscle myosins – myosin IIA and myosin IIB. GST-tagged SV1-174 (left) or GST only (right) were pre-bound to glutathione-agarose beads and incubated with the hexa-histidine (6xHis)-tagged recombinant S2 domain from the heavy chain of nonmuscle myosin IIA (S2-MHC IIA), nonmuscle myosin IIB (S2-MHC II) or skeletal muscle myosin II (S2-Sk-MHC II). Samples (15 µl) of void volumes (V, lanes 1), washes with binding buffer (W, lanes 2), washes with the indicated concentrations of NaCl in binding buffer (lanes 3-6) and eluates with 5 mM glutathione (E, lanes 7) were analyzed on immunoblots stained with anti-GST antibodies (top panels) or anti-6xHis antibodies (lower panels).





Right arrow Return to article