spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 10


Fig. 10. Pulse-chase experiments using FM4-64, LysoSensor Blue and BODIPY TR-Ceramide. (A) Pulse-chase experiments using FM4-64. (Aa,Aa') Internalized PM is accumulated in the tip region 15 minutes after the pulse. (Aa'') Five hours after the pulse, the staining in the apex was faint or absent. (Ab-Ab'') Pollen tube 24 hours after the pulse with FM4-64 (Ab); colocalization experiments using LysoSensor Blue (Ab') showed that the probes overlap in the vacuolar membranes (Ab''). (Ab''') Citofluogram that visualizes the frequency distribution of intensity in a 2D scatter plot. (B) Colocalization of FM4-64 with BODIPY-labelled membranes. (Ba,Bb) BODIPY TR-Ceramide-stained spots are distributed in the cytoplasm and are more concentrated in the tip region to form a collar-like structure (arrowheads) just behind the extreme tip at 5 (a) and 30 (b) minutes after the addition of FM4-64. (Ba',Bb') FM4-64 staining pattern after 5 (Ba') and 30 (Bb') minutes. (Ba'',Bb'') Colocalization analysis showing that probe staining overlaps in the collar-like structure and in the whole tip (white spots) after 5 and 30 minutes, respectively, after the addition of FM4-64. (Bc,Bd) Citofluograms that allow the distribution of intensity of green and red channels to be visualized in a 2D scatter plot referred to time-course analysis 5 and 30 minutes after FM4-64 addition, respectively. The ROIs select colocalized pixels. Bars, 10 µm.





Right arrow Return to article