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Figure 2


Fig. 2. Internalization of positively charged nanogold for 30 minutes. (a) After 30 minutes, positively charged nanogold (black dots) was regularly distributed on the PM and was trapped in invaginating vesicles (black arrowhead) and in vesicles immediately beneath the PM (asterisks). Vesicles of the TGN were stained (yellow arrows) as well as vesicles associated with the cis and medial rims of Golgi cisternae (G, blue arrowhead). Black arrows indicate labelled cytoplasmic vesicles. (b) A Golgi apparatus showing labelled vesicles associated with cis and medial cisternae (arrowheads). (c) Gold particles were internalized in vesicles containing cell wall components (arrowheads). (d) MVB-like compartments were labelled (arrows) as well as Golgi body vesicles (blue arrowhead). (e) A tip region showing a few stained vesicles (asterisks). (f,g) Regions far from the tip showing gold particles (black) regularly spaced on the PM surface. Bars, 0.5 µm (a,b,d-g), 1 µm (c).





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