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Figure 5


Fig. 5. Internalization of negatively charged nanogold for 15-30 minutes. (a,b) After 15 minutes, negatively charged nanogold was seen in vesicles in the TGN region (a, arrows) and in vacuole-like compartments (a, arrowheads and b, arrows). (c) After 30 minutes, invaginating vesicles were seen in the tip (arrowhead), whereas few vesicles were labelled in the apex. (d) No internalization was observed in the subapical region of the tube and Golgi (G) bodies were not labelled. Arrowhead indicates gold particles on the PM in the organelle-rich zone. (e) Organelle-rich zone showing unlabelled Golgi apparatus (G). Bars, 0.5 µm (a,b,e), 1 µm (c,d).





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