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Figure 8


Fig. 8. Time-lapse experiments. (A) Control tubes. (Aa-Aa'') green ROI indicates the area measured in the tip region. Orange and violet ROI indicate the areas measured on the flanks of tubes. (Ab) Graph showing the mean fluorescence at the tip and in the subapical regions for 300 seconds. Arrow indicates the time of FM4-64 addition. (B) Ika-treated tubes. (Ba-Ba'') Coloured areas indicate regions in which fluorescence was measured (see above). (Bb) Graph showing mean fluorescence at the tip and in subapical regions for 400 seconds. Arrow indicates time of FM4-64 addition. (Bc-Be') Medial plane images of the pollen tube 15-60 minutes after FM4-64 loading. Bars, 10 µm.





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