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Figure 9


Fig. 9. Statistical analysis of quantitative FM4-64 uptake in the tip with respect to subapical regions of pollen tubes grown in control medium and with Ika. (a) Internalization of FM4-64 in flank regions was almost double that at the apex. (b) Quantitative analysis of FM4-64 internalization in two regions of the tube, in control medium and in the presence of Ika. (a,b) Error bars indicate 95% confidence intervals (n=6). t-test analysis of FM4-64 uptake in the presence and absence of Ika gave P values in control tubes and tubes incubated with Ika that indicated that inhibition of FM4-64 uptake was significant in the apical and subapical regions (control, P=0.01401; Ika, P=0.05294). Apical (Ika) versus apical (control): P=0.01149. Subapical (Ika) versus subapical (control): P=0.00142. For quantization, the last ten frames after reaching the steady state were considered in six independent experiments.





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