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Figure 7


Fig. 7. Plasma membrane blebbing is a conserved activity of Src-kinase SH4 domains. (A) Confocal micrographs of live CHO cells stably expressing N18-HASPB-GFP (a), the indicated Src-kinase SH4 domains fused to GFP (b-e) or transiently full-length Lck fused to GFP (f). (B) Quantification of the blebbing efficiency of the cells depicted in A and in controls. Values are the arithmetic means of at least three independent experiments+s.d. In each experiment, over 100 cells were counted per condition. (C,D) SEM of CHO cells from cultures transfected with an expression construct for N18-Yes-GFP (C) or N18-Yes-{Delta}myr-GFP (D). Scale bars, 10 µm.





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