JCS007856 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 - Adobe PDF

• Supplemental Table S2 - Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Effect of forced Sox17 expression on the expression of undifferentiated-state markers. ROSA26-targeted ESCs were grown in the maintenance medium with or without Tet, and the expression of undifferentiated-state markers in these cells was evaluated by qRT-PCR. The expression levels relative to those in the parental ESCs are shown as the mean±s.d. values of three independent experiments.

• Supplemental Figure S2 -

  Fig. S2. Effect of Tet on the early and late stages of differentiation of ESCs into ExE cells. EB3 ESCs grown with or without Tet were allowed to aggregate under the same Tet status. Expression of early ExE markers in EBs grown with LIF and of late ExE markers in EBs grown without LIF was estimated by qRT-PCR. The expression levels relative to those in EBs grown without Tet are shown as the mean±s.d. values of three independent experiments.