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Figure 2


Fig. 2. Effect of forced Sox17 expression on ESC differentiation. (A) Morphology and EGFP fluorescence of ESCs grown with or without Tet. Bar, 100 µm. (B) RT-PCR analysis of ExE markers in the ESCs grown with or without Tet. cDNA derived from EBs of parental ESCs grown without LIF for various time periods was used as a positive control, and the corresponding RNA was used as the RT control. The Sox17 primer set was designed to detect both endogenous and exogenous Sox17. (C) Morphology and EGFP fluorescence of EBs. ESCs grown with or without Tet were allowed to aggregate and were grown in LIF-deficient medium with the same Tet status for 6 days. Bar, 200 µm. (D) RT-PCR analysis of germ-layer markers in EBs grown with or without Tet in LIF-deficient medium. Two independent targeted clones were examined with similar results (A-D).





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