|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 9. Inhibition of myosin II but not disruption of microtubules alters the Dia1-induced morphology of adherens junctions. (A-I) MCF7 cells transfected with GFP-mDia1 together with a vector encoding RhoA-V14. Cells were treated with DMSO (A-C) or 1 µM nocodazole (D-F) for 24 hours. The drugs were added 4 hours following transfection. Cells were stained for 
-catenin (C,F) and 
-tubulin (A,D). Cells with intact microtubules (A) display a localization of GFP-mDia1 at cell-cell junctions (B) and a significant recruitment of -catenin to the cell-cell junctions of transfected cells, as revealed by staining (C). Cells treated with nocodazole show disrupted microtubule networks (D) and a higher cytoplasmic level of GFP-mDia1, even though GFP-mDia1 still was preferentially localized to the adherens junctions (E). Disruption of the microtubule networks did not prevent, however, the increase of the recruitment of -catenin to the junctions between transfected cells (F), as compared with their non-transfected neighbors. (G-I) Cells were treated with 30 µM blebbistatin for 6 hours preceding fixation and staining with phalloidin (G) and an antibody against E-cadherin (I). GFP-mDia1 localizes to the filopodia in transfected cells (H). Arrows indicate cell-cell junctions. Bars, 10 µm.
|
