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Figure 1


Fig. 1. RPTP{alpha} is required for the reinforcement of FN-specific integrin-cytoskeleton bonds in neuronal growth cones. A laser tweezer (represented by red circle) was used to place FN-VN-coated beads at the edge of the growth cones. (A,B) In non-reinforced beads (A), the linkage between the bead and the cytoskeleton was broken by the trap force and the beads were pulled back into the trap after initial rearward movement (breaking event). If reinforcement occurred, the linkage was not broken and beads moved out of the optical trap towards the axon hillock (B). Bars, 4 µm. (C,D) FN-bead reinforcement was decreased by half but bead binding was unaffected in RPTP{alpha}–/– neurons relative to controls. By contrast, VN-bead reinforcement and binding were unaltered on RPTP{alpha}–/– neurons (C,D). Mean square displacement (MSD) was calculated for the initial 10 seconds of rearward movement for each condition. The results (mean ± s.e.) were statistically significant (P<0.01). (E,F) On average, MSD was higher for FN-coated beads bound to RPTP{alpha}–/– growth cones compared with RPTP{alpha}+/+ growth cones (E); no significant difference was observed for VN-coated beads (F).





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