spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Neurite extension on FN depends on RPTP{alpha} and matrix rigidity. (A,B) Primary neurons isolated from the hippocampi of RPTP{alpha}+/+ and RPTP{alpha}–/– mice were plated on FN-coated polyacrylamide gels of decreasing rigidities, incubated for 48 hours, fixed and visualized using phalloidin. Anti-Tau immunofluorescence was used as axonal marker. Typical (A) stage 2 and (B) stage 3 neurons are shown. Bars, 10 µm. (C,D) Control neurons showed faster differentiation on soft than on rigid substrates; RPTP{alpha}–/– neurons, however, showed a high level of differentiation irrespective of matrix rigidity (C). Similarly, control neurons extended longer neurites on soft than on rigid surfaces, whereas RPTP{alpha}–/– neurons extended long neurites on both soft and rigid surfaces (D). Results shown in C and D (mean ± s.e.) were statistically significant (P<0.01). (E,F) Phalloidin staining of RPTP{alpha}–/– and RPTP{alpha}+/+ neurons. Neurites of RPTP{alpha}–/– neurons appeared more meandering than the neurites of control neurons. Bars, 15 µm.





Right arrow Return to article