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Figure 1


Fig. 1. Cellular localisation of GFP-INPP5B. (A) HeLa cells were transiently transfected with vector encoding GFP-INPP5B, fixed in methanol, labelled with antibodies against GM130 or TGN46 and visualised by epifluorescence microscopy. (B) HeLa cells transiently expressing GFP-INPP5B were treated with 5 µg/ml nocodazole for 2 hours before labelling with antibodies to GM130, ERGIC53 or TGN46. (C,D) HeLa cells transiently expressing GFP-INPP5B were labelled with antibodies against the endosomal markers transferrin receptor (TfR) or EEA1 (C) or the ERGIC-localised proteins β-COP or ERGIC53 (D). GFP-INPP5B appears green and the other markers red in the merged images. Insets show a magnified view of the boxed areas. Bars, 10 µm.





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