spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. Golgi targeting of INPP5B requires RAB binding. (A) Sequence alignment of the OCRL1 and INPP5B linker regions showing the conservation of residues whose mutation in OCRL1 reduces RAB binding and membrane targeting (red arrows). (B) Beads containing GST-RAB5Q79L or RAB6Q72L were incubated with buffer alone or HeLa cell extract containing full-length wild-type GFP-INPP5B or the indicated point mutants and bound protein detected by western blotting with antibodies against GFP. (C) Localisation of the indicated GFP-tagged INPP5B proteins (green) in HeLa cells double-labelled with antibodies to GM130 (red). Bar, 10 µm. (D) The indicated GFP-tagged INPP5B proteins were expressed in metabolically labelled HeLa cells and immunoprecipitated with antibodies against GFP before limited digestion with the indicated amounts of trypsin. Digested proteins were analysed by SDS-PAGE and autoradiography. Markers indicate the molecular mass (kDa).





Right arrow Return to article